Dictyostelium Myosin Bipolar Thick Filament Formation: Importance of Charge and Specific Domains of the Myosin Rod

dc.contributor.authorAltman, David
dc.contributor.authorHostetter, Daniel
dc.contributor.authorRice, Sarah
dc.contributor.authorDean, Sara
dc.contributor.authorMcMahon, Peggy M.
dc.contributor.authorSutton, Shirley
dc.contributor.authorTripathy, Ashutosh
dc.contributor.authorSpudich, James A.
dc.date.accessioned2016-04-08T23:41:42Z
dc.date.available2016-04-08T23:41:42Z
dc.date.issued2004-11
dc.description.abstractMyosin-II thick filament formation in Dictyostelium is an excellent system for investigating the phenomenon of self-assembly, as the myosin molecule itself contains all the information required to form a structure of defined size. Phosphorylation of only three threonine residues can dramatically change the assembly state of myosin-II. We show here that the C-terminal 68 kDa of the myosin-II tail (termed AD-Cterm) assembles in a regulated manner similar to full-length myosin-II and forms bipolar thick filament (BTF) structures when a green fluorescent protein (GFP) "head" is added to the N terminus. The localization of this GFP-AD-Cterm to the cleavage furrow of dividing Dictyostelium cells depends on assembly state, similar to full-length myosin-II. This tail fragment therefore represents a good model system for the regulated formation and localization of BTFs. By reducing regulated BTF assembly to a more manageable model system, we were able to explore determinants of myosin-II self-assembly. Our data support a model in which a globular head limits the size of a BTF, and the large-scale charge character of the AD-Cterm region is important for BTF formation. Truncation analysis of AD-Cterm tail fragments shows that assembly is delicately balanced, resulting in assembled myosin-II molecules that are poised to disassemble due to the phosphorylation of only three threonines.en_US
dc.identifier.citationHostetter, Daniel, et al. "Dictyostelium myosin bipolar thick filament formation: importance of charge and specific domains of the myosin rod." PLoS Biol 2.11 (2004): e356.en_US
dc.identifier.issn1544-9173
dc.identifier.urihttp://hdl.handle.net/10177/5351
dc.language.isoen_USen_US
dc.publisherPublic Library of Scienceen_US
dc.titleDictyostelium Myosin Bipolar Thick Filament Formation: Importance of Charge and Specific Domains of the Myosin Roden_US
dc.typeArticleen_US

Files

Original bundle
Now showing 1 - 1 of 1
Loading...
Thumbnail Image
Name:
pbio.0020356.pdf
Size:
593.74 KB
Format:
Adobe Portable Document Format
Description:
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.51 KB
Format:
Item-specific license agreed upon to submission
Description: